Metagenomics a Multifurcated Approach to Retrieve the Untapped Resources

EasyChair Preprint 14610

Abstract

Metagenomic studies facilitate the detection of microbial communities without culturing of bacteria; in addition, direct molecular methods preserve the in situ metabolic status and microbial community composition-because samples are frozen immediately after acquisition. Also direct extraction of nucleic acids from experimental samples accounts for the very large proportions of microorganisms (90-99.9%) that are not readily cultured in the laboratory, but that may be responsible for the majority of the biodegradable activity of interest. The development of metagenomics and the application of high throughput technologies for handling and screening of very large numbers of clones represent important factors to increase speed and efficacy of enzyme discovery, thus accelerating and broadening the establishment of biocatalytic applications. In addition to application-oriented benefits, phylogenetic analysis of novel genes could also provide insight into evolutionary affinity, natural distribution and development of distinct enzymatic activities and could serve to elucidate evolutionary structure-function relationships. When combined with classic microbiological methods, these molecular biological methods and metagenomics approach will pave a new way to explore novel catabolic genes responsible for degradation of toxic pollutants.

Keyphrases: Applied microbiology and biotechnology, Ion torrent platform, Nucleic Acids Research, applied and environmental microbiology, bacterial community, bacterial diversity, meta-data analysis, metagenomics, microbial communities, microbial community analysis, microbial diversity, next generation sequencing, understanding of microbial diversity

@booklet{EasyChair:14610,
  author    = {Jeyaraj Pandiarajan and Muthukalingan Krishnan and Thangaiyan Suganya and Karuppasamy Muthuvel Prasath},
  title     = {Metagenomics a Multifurcated Approach to Retrieve the Untapped Resources},
  howpublished = {EasyChair Preprint 14610},
  year      = {EasyChair, 2024}}